LOCUS       pLV-[hsa-miR-486]       9122 bp    DNA     circular     2-MAR-2011
SOURCE      
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7043..7903
                     /label=Amp(R)
     promoter        8104..8644
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5648..5882
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6457..6912)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5954..6084
                     /label=SV40 pA
     misc_feature    6085..6298
                     /label=SV40 ori
     misc_feature    4969..5565
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3587
                     /label=intron
     CDS             3634..4959
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3377
                     /label=hsa-miR-486 precursor
BASE COUNT     2146 a      2396 c      2489 g      2091 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc ggtcccagag 
     3121 tcaggccctg gggtgtgaat ggtaacggcc tggttagtgc tgcactgggg ccaccatgcc 
     3181 ccctcacctc ctgctgactc ccaccccgag gtatcctgta ctgagctgcc ccgagctggg 
     3241 cagcatgaag ggcctcgggg cagctcagta caggatgccc cagggaggat ggagatcaga 
     3301 gccggccgct gaagccccca gcaccacaca cacctgctgg agatggtcgc acagcgtctt 
     3361 cagtcccttc ctcatgggct agctcgagct tttggagtac gtcgtcttta ggttgggggg 
     3421 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag 
     3481 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca 
     3541 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaaaac 
     3601 tacccctcta gagtcgagct accggtcgcc accatggtga gcaagggcga ggaggataac 
     3661 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 
     3721 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 
     3781 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 
     3841 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 
     3901 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 
     3961 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 
     4021 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 
     4081 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 
     4141 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 
     4201 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 
     4261 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 
     4321 ggcatggacg agctgtacaa ggacccaccg gtcgccacca tgaccgagta caagcccacg 
     4381 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 
     4441 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 
     4501 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 
     4561 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 
     4621 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 
     4681 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 
     4741 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 
     4801 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 
     4861 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac 
     4921 cgcgcgacct ggtgcatgac ccgcaagccc ggtgcctgag cggccgcgtc gacaatcaac 
     4981 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 
     5041 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 
     5101 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 
     5161 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 
     5221 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 
     5281 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 
     5341 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 
     5401 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 
     5461 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 
     5521 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt 
     5581 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 
     5641 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 
     5701 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 
     5761 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 
     5821 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 
     5881 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 
     5941 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     6001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     6061 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 
     6121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     6181 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     6241 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 
     6301 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
     6361 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
     6421 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 
     6481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     6541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     6601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     6661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     6721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     6781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     6841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     6901 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     6961 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     7021 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     7081 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     7141 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     7201 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     7261 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     7321 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     7381 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     7441 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     7501 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     7561 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     7621 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     7681 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     7741 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     7801 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     7861 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     7921 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     7981 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     8041 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     8101 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     8161 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     8221 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     8281 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     8341 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     8401 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     8461 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     8521 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     8581 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     8641 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     8701 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     8761 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
     8821 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     8881 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     8941 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
     9001 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
     9061 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 
     9121 gc 
//
